Snapgene download serial#

Many of the above-listed plasmid mapping tools have built-in primer design tools, but here are a few stand-alone sites for general primer design, as well as primer design for different cloning applications. Filter DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications.DNA sequence reverse complement tool: Enter a DNA sequence and the program will give you the reverse sequence, complement sequence, or reverse and complement sequence.

Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them.

Codon optimization tool: To access this tool, set up an IDT account.įigure 2: Addgene's Analyze Sequence tool.

UniProt: A database of protein sequence and functional information, such as expression patterns, post-translational modifications and polymorphisms.

ExPASy translation tool: Translates a DNA sequence using all six open reading frames.

NCBI BLAST: Compares nucleotide or protein sequences to sequences in a database.Addgene vector database: Find sequences for plasmids assembled from publications and commercially available sources, as well as those deposited with Addgene.Webcutter 2.0: For finding restriction enzyme cut sites.NEBCutter2: For finding restriction enzyme cut sites.Addgene's analyze sequence tool: Annotates features, BLASTs, aligns, digests, and translates a sequence.Wayne Davis from the University of Utah.įigure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.ĭNA / protein database search and analysis tools ApE (A plasmid Editor): A free, donation-based plasmid analysis tool including editing, annotating, creating maps, and more.

Snapgene download serial#

Serial Cloner: Free desktop-based software for plasmid design and mapping.Benchling: While you might think of Benchling as an electronic lab notebook, it also has a suite of molecular biology tools and can make plasmid maps.SnapGene Viewer: The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, while the paid version provides more tools for plasmid mapping and design (Figure 1).These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate any DNA sequence. To help you keep your virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology tools all in one place. We all have our favorite tools for tackling these particular tasks, but they tend to be scattered about the internet. Prices start at $295.00 per year and up.Primer design. Not all features available in SnapGene Viewer. Freely share data with your colleagues or customers using the universally accessible SnapGene format.ģ0-Day Trial.Export a plasmid map as an image, or export an annotated DNA sequence to GenBank format.Identify open reading frames (ORFs) with a mouse click.Design and annotate primers for PCR, sequencing, or mutagenesis.Automatically annotate common features or manually annotate coding sequences and other features.Search a DNA sequence to match either a DNA query, a protein translation, or an annotation.Browse or print a DNA sequence and its annotations using customizable Map, Sequence, Enzymes, Features, Primers, and History views.Create a DNA sequence file by either entering a sequence, importing a record from GenBank, or opening an annotated sequence stored in one of many common file formats.SnapGene Viewer allows molecular biologists to create, browse, and share richly annotated DNA sequence files up to 1GB in length.